Statistical Optimisation of Used-Cooking-Oil Degradation by Burkholderia vietnamiensis AQ5-12 and Burkholderia sp. AQ5-13

Used cooking oil (UCO) is a hydrocarbon that causes significant environmental pollution globally. Oil difficult pollutant to remove from the environment due its hydrophobic nature. Bioremediation preferred over chemical and physical remediation approaches as it more economical environmentally friendly. Hence, search for microbe with outstanding degrading capability imperative nature of hydrocarbons pollutants. The current study evaluates ability glyphosate degrader isolates, Burkholderia vietnamiensis AQ5-12 sp. AQ5-13, degrade UCO sole carbon source. physico-cultural conditions affecting activities isolates were studied using one-factor-at-a-time (OFAT) response-surface methodology (RSM). temperature, agitation speed, concentration, nitrogen supply, yeast extract inoculum size optimised during 7-day incubation period. Based on OFAT results, highest degradations 62.23% 58.33% respectively. optimisation RSM showed 83.42% 87.09% degradation by AQ5-13 isolates. Parameters including extract, ammonium sulphate concentration provided interaction model in isolate AQ5-12. Meanwhile, significance all five parameters: pH, concentration. efficiency validated via demonstrated better results than approach. validation has verified no difference between experimental predicted values. these two bacterial worthwhile procedure bioremediation hydrocarbon-rich areas.